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- W2393278329 abstract "[Objective]To investigate the effects of apigenin on proteoglycan synthesis in the human lumbar intervertebral disc in response to hydrostatic pressure.[Method]Thirty-two lumbar intervertebral disc were obtained from patients who had undergone posterior discectomy.These samples were chopped into 1-2 mm3 and incubated in a 2.5 ml's plastic syringe with 1 ml Dulbecco's Modified Eagle's Medium(DMEM).Apigenin,L-NMMA and SNAP were added into the DMEM,cultured for 2 hours in the hydrostatic pressure devices,and then measured the proteoglycan content in the supernatant by means of Coomassie brilliant blue albument ration.[Result]Apigenin 3 atm group and L-NMMA 3 atm group had the highest rate of proteoglycan synthesis,there were no significant difference between the two groups(P0.05),there was significant difference compared with control group(P0.05)(however,they were siginificantly higher than that of control group).SNAP 30atm group had the lowest rate of proteoglycan synthesis,significant differences were found when compared with the various groups(P0.01).In control group,L-NMMA group,SNAP group and apigenin group under the 3 atm's hydrostatic pressure proteoglycan synthesis rates were significantly higher than under the 30 atm's,there were significant difference compared with it(P0.05).[Conclusion] Apigenin can increase proteoglycan synthesis ratio of the intervertebral disc under 3atm's hydrostatic pressure,and reduce the 30atm's hydrostatic pressure's inhibition on proteoglycan synthesis ratio." @default.
- W2393278329 created "2016-06-24" @default.
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- W2393278329 date "2012-01-01" @default.
- W2393278329 modified "2023-09-24" @default.
- W2393278329 title "The effects of apigenin on proteoglycan synthesis in the human lumbar discs under hydrostatic pressure" @default.
- W2393278329 hasPublicationYear "2012" @default.
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