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- W2393285866 abstract "Purpose To establish a FQ?PCR (fluorogenic quantitative RT?PCR) method for detecting the expression of tissue factor gene in tissue and investigate the relationship between TF mRNA expression and tumor. Methods Fluorogenic quantitative RT?PCR was set up to examining the specific expression of tissue factor in 42 glioma tissues,5 normal brain tissue and glioma cell line U?251. Results In 42 glioma tissues TF mRNA expression ranges from 3.6×10 5-8.8×10 7 copy/μg RNA,mean value is 2.9×10 6 copy/μg RNA.TF mRNA expression level correlates with the histologic grade of malignancy.In one of 5 normal brain specimens,TF mRNA is under detection limit whereas in the other 4 normal specimens TF mRNA expression level is from 3.8×10 3-5.1×10 4 copy/μg RNA,mean value is 6.2×10 3 copy/μg RNA.For glioma cell line U?251 the expression level is 2.8×10 6 copy/μg RNA. Conclusions Tissue factor gene expression detected with FQ?PCR had an accurate,speedy results.TF mRNA may be used as a valuable marker for evaluating the grade of glioma malignancy." @default.
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- W2393285866 date "2002-01-01" @default.
- W2393285866 modified "2023-09-25" @default.
- W2393285866 title "Quantification of Tissue Factor Gene Expression using Fluorogenic Quantitative RT-PCR Method" @default.
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