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- W2393297290 abstract "In this report, we studied the effects of bone marrow stromal cell line QXMSCj on the CFU-F, CFU-GM, BFU-E and CFU-E. The mechanism of improving hematopoiesis by stromal cells was investigated by establishing long-term coculture system of QXMSQ stromal cells with the hematopoietic cells of bone marrow. The results indicated that the cultural supernatant of QXMSQ cells could significantly stimulated the growth of CFU-F, CFU-GM, BFU-E and CFU-E. QXMSQ cells could also maintain the growth of CFU-GM, BFU-E and CFU-E with conditioned medium lacking Epo, implying QXMSC1 cells secreted GM-CSF and Epo. In the coculture system, QXMSQ is able to increase the number of CFU-GM. The millipore membrane was used to separate the QXMSQ cells from hematopoietic cells in the coculture, so as to see the way by which the QXMSQ stimulates CFU-GM. The result indicated that the separated QXMSC1 stromal cells are able to support the growth of CFU-GM progenitor, but lower than the contact coculture, implying that the QXMSC1 stromal cell can improve the hematopoiesis by direct contact with hematopoietic cells and secreting cytokines." @default.
- W2393297290 created "2016-06-24" @default.
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- W2393297290 date "1997-01-01" @default.
- W2393297290 modified "2023-09-25" @default.
- W2393297290 title "Effect of Bone Marrow Stromal Cell Line QXMSC_1 on Hematopoietic Regulation In Vitro" @default.
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