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- W2393365079 abstract "Objective To purify the fusion protein His-KGPcd(gingipain K catalytic domain) from Porphyromonas gingivalis and prepare the polyclonal anti-KGPcd antibody.Methods The gene fragment encoding kgpcd was inserted into prokaryotic expression vector pET-16b.The expressed fusion protein was purified by Ni-NTA affinity chromatography and then renatured by dilution and dialysis.Then the fusion protein was characterized by Western blot.The recombined and purified KGPcd protein combined with the complete Freund′s adjuvant was used to immunize the rabbits.Then the anti-KGPcd antibody was prepared.The antisera were identified by indirect ELISA.Results The fusion protein His-KGPcd was gotten through purification by Ni-NTA affinity chromatography.The antisera could be combined with the KGPcd specifically.Conclusion The polyclonal anti-KGPcd antibody was successfully prepared in this study." @default.
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- W2393365079 date "2011-01-01" @default.
- W2393365079 modified "2023-09-23" @default.
- W2393365079 title "Purification of the fusion protein KGPcd and preparation of the polyclonal anti-KGPcd antibody" @default.
- W2393365079 hasPublicationYear "2011" @default.
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