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- W2393370042 abstract "Objective To investigate the feasibility of electrospun collagen/PLCL composite nanofibrous membranes combined with costal chondrocytes to construct tissue-engineered tracheal patch.Methods Costal chondrocytes were isolated and expanded to two passages from New Zealand rabbits,seeded on the collagen/PLCL nanofibrous scaffolds for scanning electron microscopy.Then,the cell-scaffold compounds were constructed by a sandwich model,cultured in vitro for 4 weeks and subsequently implanted subcutaneously in nude mouse for additional 4 weeks.The cartilage-like tissues were evaluated by gross view,histology staining and typeⅡcollagen immunohistochemistry.Results We found that the scaffold facilitated the proliferation of costal chondrocytes from the results of scanning electron microscopy.The cell-scaffold compounds were similar to cartilage tissues after cultured in vivo for additional 4 weeks.HE staining proved amount cartilage lacuna formation,while the secretion of cartilage matrix were proved by toluidine blue and safranin O.Besides,immunohistochemistry proved the expression of typeⅡcollagen.Conclusions Electrospun collagen/PLCL composite nanofibrous membranes had the satisfactory biocompatibility and were considered as the suitable scaffolds for tissue-engineered tracheal patch." @default.
- W2393370042 created "2016-06-24" @default.
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- W2393370042 date "2012-01-01" @default.
- W2393370042 modified "2023-09-23" @default.
- W2393370042 title "Preliminary study on construction of tissue-engineered tracheal patch by electrospun collagen/PLCL composite nanofibrous membranes" @default.
- W2393370042 hasPublicationYear "2012" @default.
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