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- W2393375127 abstract "Objective To investigate the current status of domestic radioimmunoassay(RIA) measurement systems for serum insulin.Methods The serum insulin levels in 140 patients were measured by using four kinds of domestic radioimmunoassay kits and E170 electrochemiluminescence system respectively.The measured results of serum insulin levels were divided into four groups according to the results of E170.The detection of insulin levels in two serum samples mixed from lowest and highest groups were repeated for 20 times by using four domestic RIA kits.The intra-batch coefficient of variability(CV%) was also calculated.The other 68 serum samples with insulin levels less than 10 μIU/mL determined by E170 were measured by the domestic kit A,and then the linear regression was calculated between these two measurement systems.The data were analysed by using paired t-test.Results The significant differences among the four kinds of domestic kit were observed in all four levels of serum insulin by ANOVA analysis.The F value was 6.7,13.8,9.6 and 9.2 respectively(P0.001).The intra-batch CV% for all the four kinds of domestic kit was within 10%.Compared with insulin level determined by E170,the corrected value for domestic kit A were not significantly different(P0.05),the correlation coefficients before and after the correction were 0.49.Conclusion As the incomparability and the intractability exist in four kinds domestic RIA measurement systems,establishing a reference system to improve the accuracy is necessary." @default.
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- W2393375127 date "2012-01-01" @default.
- W2393375127 modified "2023-09-25" @default.
- W2393375127 title "Analysis of Domestic Radioimmunoassay Measurement Systems" @default.
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