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- W2393375997 abstract "Employing PCR method, the cloned grass carp IGF Ⅰgene was modified to be a mature IGF I peptide gene and then subcloned into fusion expression vector pGEX 4T 1 to construct expression vector pGEX IGF. Restriction endonuclease analysis and sequencing confirmed the corrected construction. The vector was transformed into Escherichia coli BL21 cell which can be induced by IPTG to produce a special protein of 34kD in molecular weight. The special protein can be mainly in form of soluble protein or inclusion body when induced at 30 and 37℃ respectively. The cells induced at 30℃ were digested by lysozmye and disrupted by sonication. After being centrifuged the supernatant was purified by glutathione sepharose column. GST IGF fusion protein could be absorbed specifically on this column. The high purified protein was used as antigen to immune rabbits directly and high quality antiserum was obtained, which showed that the fusion protein possesses good immunogenicity." @default.
- W2393375997 created "2016-06-24" @default.
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- W2393375997 date "2002-01-01" @default.
- W2393375997 modified "2023-10-04" @default.
- W2393375997 title "Fusion expression and purification of Ctenopharyngodon idellus IGF-I and preparation of the antiserum against GST-IGF" @default.
- W2393375997 hasPublicationYear "2002" @default.
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