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- W2393398985 abstract "The on-line stacking effect of benzaldehyde in high performance liquid chromatography(HPLC) based on the velocity difference of the solute on the different stationary phase was discussed by the coupling of two different stationary phases,using H2O and acetonitrile as mobile phase.The effective stacking was observed and the stacking factor increased with the increase of the velocity difference(the ratio of v1 to v2).Under the optimal experimental conditions,the stacking factor was 4.6,and the peak height increased 5.4-fold.The RSD of retention time,peak area,peak height and peak width at half peak height was 1.2%,4.2%,3.4% and 0.80%,respectively.Successful stacking was observed by the application of this stacking effect to the real sample in the monoamine oxidase(MAO) activity determination and the signal/noise(S/N) ratio increased 6.9-fold.In real sample determination,the RSD of retention time,peak area,peak height,peak width at half peak height,plate number and S/N was 0.12%,4.59%,5.01%,0.70%,1.24% and 4.65%,respectively,which indicated that the stacking effect can be successfully used in the real sample determination with improved sensitivity." @default.
- W2393398985 created "2016-06-24" @default.
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- W2393398985 date "2009-01-01" @default.
- W2393398985 modified "2023-09-28" @default.
- W2393398985 title "On-Line Stacking Effect of Benzaldehyde in High Performance Liquid Chromatography and Its Application" @default.
- W2393398985 hasPublicationYear "2009" @default.
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