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- W2393405665 abstract "Objective To establish mouse embryonic fibroblasts(MEFs) as the feeder layers for supporting induced pluripotent stem cells(iPSCs) in vitro.Methods The mouse primary embryonic fibroblasts were isolated from the mouse embryos of 13.5-15.5 day gestation through primary tissue digestion.The morphology and growth curve of MEFs in vitro was investigated.And then the second or third passage of MEFs was treated with 10μg/ml mitomycin C for 2-3 hours to prepare fibroblast feeder layers.IPSCs were cultured on the feeder layer and iPSCs growth was observed.The expression of alkaline phosphatase(AKP) of iPSCs was tested.Results After treated by mitomycin C,MEFs had the capability to secrete multiple growth factors,neither proliferation nor apoptosis.iPSCs were cultured on the feeder layer grew well and maintained undifferentiation and vitality,which also could form the nest morphology as embryonic stem cells clone and show the positive result of AKP.Conclusion Preparing MEFs as feeder layers with the measure mentioned about can be used for culturing iPSCs in vitro." @default.
- W2393405665 created "2016-06-24" @default.
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- W2393405665 date "2011-01-01" @default.
- W2393405665 modified "2023-09-28" @default.
- W2393405665 title "Study on preparation of feeder layers for induced pluripotent stem cells" @default.
- W2393405665 hasPublicationYear "2011" @default.
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