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- W2393432075 abstract "Objective:To characterize B cell antigenic epitopes of different genotypes of hepatitis E virus (HEV) with monoclonal antibodies (McAbs) against HEV ORF2-encoded proteins (p166) of the HEV Burma and Mexico strains.Methods:The recombinant proteins (p166Bur and p166Mex) were used to immunize BALB/C mice,respectively. Spleen cells of the immunized mice were fused with SP2/0 myeloma cells. Hybridoma clones which secreted McAbs against p166Bur or p166Mex were screened by an enzyme-linked immunosorbent assay (ELISA). Finally, different p166 recombinant proteins (p166Bur, p166Pak, p166Mor, p166Mex, p166Us, p166Nz and p166Ch) obtained from HEV genotype Ⅰ,Ⅱ,Ⅲ or Ⅳ were tested with the generated McAbs by ELISA and Western blotting.Results:Four hybridoma cell lines, numbered 2C 2, 2B 1 and D 8G 10, E 5E 12 were obtained from the spleen cells of mice immunized with p166Bur and p166Mex, respectively. McAb produced by 2B 1 reacted with p166Bur, p166Pak, p166Mor and p166Mex, which derived from HEV genotypeⅠand Ⅱ, but did not react with p166Us, p166Nz and p166Ch, which derived from HEV genotype Ⅲ and Ⅳ. However, McAbs produced by 2C 2 , D 8G 10 and E 5E 12 were reactive to the p166 proteins prepared not only from genotype Ⅰ and Ⅱ, but also from genotype Ⅲ and Ⅳ.Conclusion:Both common and different antigenic epitopes within p166 recombinant proteins exist between HEV genotype Ⅰ, Ⅱ and genotype Ⅲ,Ⅳ." @default.
- W2393432075 created "2016-06-24" @default.
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- W2393432075 date "2004-01-01" @default.
- W2393432075 modified "2023-09-25" @default.
- W2393432075 title "Comparison of B cell antigenic epitopes between hepatitis E virus genotypeI,II and genotype III,IV by using monoclonal antibodies" @default.
- W2393432075 hasPublicationYear "2004" @default.
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