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- W2393480275 abstract "Based on the published sequence of F gene of Newcastle disease virus strain SX-1 isolated from spotted chicken,through PCR site-directed mutagenesis in vitro,two pairs of primers were designed,which contained mutation sites. Overlap PCR was used three times,then obtained the mutational F gene,which was named F′ gene,and the amino acid sequence in the cleavage site was 112Gly-Arg-Gln-Gly-Arg-Leu117. Then,F′ gene and the vector pfastBacⅠwere connected by EcoRⅠand HindⅢ sites. After identification by PCR,restriction enzyme digestion and sequencing,the positive recombinant which designated as pFastBac-F′ was transformed into the host cell DH10Bac to construct the recombinant plasmids Bacmid F′. At last,the Bacmid F′ was transfected into Sf-9 cells for the expression of F′ gene. Indirect immunofluorescence saasy,SDS-PAGE and Western-blotting were used to identify the expression,and the results showed that F′ recombinant protein was expressed well." @default.
- W2393480275 created "2016-06-24" @default.
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- W2393480275 date "2011-01-01" @default.
- W2393480275 modified "2023-09-23" @default.
- W2393480275 title "Point Mutation and Eukaryotic Expression of F Gene of Newcastle Disease Virus Isolated from Spotted Chicken" @default.
- W2393480275 hasPublicationYear "2011" @default.
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