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- W2393486445 abstract "Objective:To construct a brain cDNA library in morphine dependent rats.Methods:The morphine dependent rat brain was extracted by decapitation.Total RNA was isolated from the rat brain by TRIzol and poly A+RNA was pruified subsequently from total RNA.The mRNA was used as starting material in first-strand synthesis by reverse transcription and then cDNA was synthesized by LD-PCR.After being digested by SfiⅠA and B,the ligated cDNA and pTRG vector was transformed into the XL1-Blue MRF′ Kan competent cell.Results and Conclusion:The results of PCR indicated that ligated cDNA was about 1 kb and the ligation rate was above 90%.The morphine dependent rat brain library contained 3.05×106 clones and could be used for bacterial two-hybrid screening." @default.
- W2393486445 created "2016-06-24" @default.
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- W2393486445 date "2007-01-01" @default.
- W2393486445 modified "2023-09-27" @default.
- W2393486445 title "Brain cDNA library construction in morphine dependent rats" @default.
- W2393486445 hasPublicationYear "2007" @default.
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