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- W2393522016 abstract "OBJECTIVE To establish a method for determination of the contents of rutin and quercetin in Ocimi Herba,and to determine the contents of rutin and quercetin in Ocimi Herba from different places.METHODS The contents of rutin and quercetin in Ocimi Herba were determined by HPLC.The rutin was determined by using mobile phase consisted of acetonitrile-0.4% phosphoric acid solvent(18∶82),the quercetin was determined by using mobile phase consisted of acetonitrile-0.4% phosphoric acid solvent(32∶68).All the UV wavelength was 360 nm,the flow rate was 1.0 mL·min.1 and the column temperature was 30 ℃.RESULTS The linear range of rutin was 4.952-4.750 μg(r=0.999 7) with a mean recovery of 100.7% and RSD 1.62%(n=6).The linear range of quercetin was 1.792.8.064 μg(r=0.999 7) with a mean recovery of 98.3%,RSD 0.94%(n=6).The contents of rutin and quercetin in Ocimi Herba from different places were within the range of 0.39.0.71 mg·g.1 and 0.29.0.58 mg·g.1.CONCLUSION The method is specific,reliable and accurate.It can be used for the quality control of Ocimi Herba effectively." @default.
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- W2393522016 date "2011-01-01" @default.
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- W2393522016 title "Determination of Rutin and Quercetin in Ocimi Herba from Different Places by HPLC" @default.
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