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- W2393553114 abstract "Endophytes-infected grasses have an extraordinary impact on the ecology and economy of pasture and turf.Festuca arundinacea and Lolium perenne infected Neotyphodium coenophialum and N.lolii are toxic to livestock fed on them.N.coenophialum and N.lolii in the imported grass seeds of Festuca arundinacea and Lolium perenne by Tianjin Port since 1998 were microscopically affirmed to take endophytes and were separated and cultivated.The purity and concentration of the genomic DNA extracted from hyphaes of 12 suspected strains with improved Moller,et al.method was measured by ultraviolet spectroscopy.The purity and the concentration increased slightly in contrast with the Moller,et al.method.PCR products with primers IS1-IS3 according to Tubulin2 appeared to be a single band respectively through gel electrophoresis,and sequence analysis united with morphologic proofs showed that the suspected strains could be confirmed to be N.coenophialum and N.lolii respectively.The primer F1-R1 of NC25 gene had designed according to sequences from Genbank,and the PCR result showed that N.coenophialum was evidently different from N.lolii by gel electrophoresis,since the product of N.coenophialum was 160 bp larger than that of N.lolii by amplification with the same primer F1-R1.The detection based on PCR is accurate and credible." @default.
- W2393553114 created "2016-06-24" @default.
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- W2393553114 date "2006-01-01" @default.
- W2393553114 modified "2023-09-23" @default.
- W2393553114 title "Optimization of extraction of mycelial genomic DNA of Neotyphodium coenophialum and N.lolii and their preliminary detection based on PCR" @default.
- W2393553114 hasPublicationYear "2006" @default.
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