Matches in SemOpenAlex for { <https://semopenalex.org/work/W2393569190> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2393569190 abstract "Objective To filtrate simple effective method separating and purifying manganese porphyrin triple helix forming oligonucleotide (TFO) acridine compounds. Method TFO modified by acridine and amino modifier C6 were synthesized by solid phase phosphoramidite chemistry, then deprotected mildly. Meso tetrakis(4 carbocyphenyl) porphyrin was prepared by metallation and esterification, then linked with previously described oligonucleotides. The reaction products were separated and purified by denaturing polyacrylamide gel, oligonucleotide purification cartridge and thin layer plate respectively. Results When the reaction products were separated by denaturing polyacrylamide gel, the reaction products strand and manganese porphyrin activated esters strand were at same level, manganese porphyrin TFO acridine compounds strand was not observed. After the reaction products were separated by oligonucleotide purification cartridge, the products were still mixed of manganese porphyrin TFO acridine compounds and manganese porphyrin activated esters, which was made sure by ultraviolet absorption spectrometry. When the reaction products were separated by thin layer plate, there was a green lunarform strand behind manganese porphyrin monoactivated esters, the reclaim of this strand possess characteristic ultraviolet absorption peak of oligonucleotide and manganese porphyrin, which was show by ultraviolet absorption spectra. Mass spectrogram confirmed that the observed mass of TFO derivative were same as that of the theoretical mass. Conclusion Manganese porphyrin TFO acridine compounds were separated and purified simply and effectively by thin layer plate." @default.
- W2393569190 created "2016-06-24" @default.
- W2393569190 creator A5055328507 @default.
- W2393569190 date "2002-01-01" @default.
- W2393569190 modified "2023-09-25" @default.
- W2393569190 title "Purification of manganese porphyrin-TFO with thin layer chromatography" @default.
- W2393569190 hasPublicationYear "2002" @default.
- W2393569190 type Work @default.
- W2393569190 sameAs 2393569190 @default.
- W2393569190 citedByCount "0" @default.
- W2393569190 crossrefType "journal-article" @default.
- W2393569190 hasAuthorship W2393569190A5055328507 @default.
- W2393569190 hasConcept C129312508 @default.
- W2393569190 hasConcept C178790620 @default.
- W2393569190 hasConcept C185592680 @default.
- W2393569190 hasConcept C190196608 @default.
- W2393569190 hasConcept C2776043002 @default.
- W2393569190 hasConcept C2776924795 @default.
- W2393569190 hasConcept C2779480358 @default.
- W2393569190 hasConcept C43617362 @default.
- W2393569190 hasConcept C528890316 @default.
- W2393569190 hasConcept C552990157 @default.
- W2393569190 hasConcept C55493867 @default.
- W2393569190 hasConcept C75473681 @default.
- W2393569190 hasConceptScore W2393569190C129312508 @default.
- W2393569190 hasConceptScore W2393569190C178790620 @default.
- W2393569190 hasConceptScore W2393569190C185592680 @default.
- W2393569190 hasConceptScore W2393569190C190196608 @default.
- W2393569190 hasConceptScore W2393569190C2776043002 @default.
- W2393569190 hasConceptScore W2393569190C2776924795 @default.
- W2393569190 hasConceptScore W2393569190C2779480358 @default.
- W2393569190 hasConceptScore W2393569190C43617362 @default.
- W2393569190 hasConceptScore W2393569190C528890316 @default.
- W2393569190 hasConceptScore W2393569190C552990157 @default.
- W2393569190 hasConceptScore W2393569190C55493867 @default.
- W2393569190 hasConceptScore W2393569190C75473681 @default.
- W2393569190 hasLocation W23935691901 @default.
- W2393569190 hasOpenAccess W2393569190 @default.
- W2393569190 hasPrimaryLocation W23935691901 @default.
- W2393569190 hasRelatedWork W1415683861 @default.
- W2393569190 hasRelatedWork W1975464372 @default.
- W2393569190 hasRelatedWork W1984769318 @default.
- W2393569190 hasRelatedWork W1987646266 @default.
- W2393569190 hasRelatedWork W1992169657 @default.
- W2393569190 hasRelatedWork W1999896285 @default.
- W2393569190 hasRelatedWork W2023769605 @default.
- W2393569190 hasRelatedWork W2039160967 @default.
- W2393569190 hasRelatedWork W2082208231 @default.
- W2393569190 hasRelatedWork W2082691593 @default.
- W2393569190 hasRelatedWork W2083239479 @default.
- W2393569190 hasRelatedWork W2096121560 @default.
- W2393569190 hasRelatedWork W2109802345 @default.
- W2393569190 hasRelatedWork W2258710345 @default.
- W2393569190 hasRelatedWork W2777752457 @default.
- W2393569190 hasRelatedWork W38886951 @default.
- W2393569190 hasRelatedWork W882022247 @default.
- W2393569190 hasRelatedWork W2922814162 @default.
- W2393569190 hasRelatedWork W2926713841 @default.
- W2393569190 hasRelatedWork W3001368528 @default.
- W2393569190 isParatext "false" @default.
- W2393569190 isRetracted "false" @default.
- W2393569190 magId "2393569190" @default.
- W2393569190 workType "article" @default.