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- W2393581489 abstract "Objective:To prepare the expressed DNA fragment for the construction of anti-caries DNA vaccine. Methods:To confirm that pPC41,a recombinant plasmid,contains pac gene by way of restriction enzyme digestion and electrophoresis;To choose important regions in pac gene and to design primers with computer, and then to amplify DNA sample. Restriction enzyme digestion and electrophoresis were used to identify PCR products. Results:pPC41 was verified to contain pac gene.The DNA fragment amplified from pac gene was expected one. Conclusion:The successful PCR amplification to important regions in pac gene is a significant preparation for the construction of recombinant anti-caries vaccine. [" @default.
- W2393581489 created "2016-06-24" @default.
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- W2393581489 date "2000-01-01" @default.
- W2393581489 modified "2023-09-25" @default.
- W2393581489 title "Design and preparation of cloned DNA fragment from pac gene of Streptococcus mutans" @default.
- W2393581489 hasPublicationYear "2000" @default.
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