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- W2393614269 abstract "Objective To clone the surface antigen(SAG3)gene of Toxoplasma gondii and analyze structure and function of SAG3 protein.Methods The SAG3 gene was amplified from the total RNA of Toxoplasma gondii by RT-PCR.Meanwhile,the physicochemical characteristics,structure and function of SAG3 protein was analyzed and predicted by bioinformatics software.Results The amplifed gene fragment was 1 158 bp,in accordance with the target gene.Analysis of the predicted protein indicated a molecular mass of 41.773 2 ku,two conserved region and function sites.Predicted PI 6.75 suggested SAG3 protein was amphipathic.Conclusion SAG3 gene is successfully cloned,which may lay a solid foundation for exploring its structure and function." @default.
- W2393614269 created "2016-06-24" @default.
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- W2393614269 date "2008-01-01" @default.
- W2393614269 modified "2023-09-28" @default.
- W2393614269 title "Cloning and bioinformatics analysis of SAG3 gene from Toxoplasma gondii" @default.
- W2393614269 hasPublicationYear "2008" @default.
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