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- W2393625692 abstract "To determine the role of the new gene cglE of Escherichia coli causing meningitis to penetrate across the blood-brain barrier,this gene was firstly amplified by PCR,subcloned into prokaryotic expression vector pET22b,and then it was transformed to E.coli BL21(DE3).The recombinant fusion protein CglE was expressed in E.coli BL21 cells after induction with IPTG.Meanwhile,bioinformatics was used to analyze the primary and secondary structures of CglE protein.It appeared that the CglE fusion protein identified by Wstern blot analysis was sized about 50 000 in relative molecular mass as demonstrated by SDS-PAGE.The result in the bioinformatics analysis indicated that this gene shared sequence homology with gene encoding dihydrolipoamide dihydrogenase(DLDH) of various species of bacteria,and the sequence of CglE protein showed similarity in secondary structures with the invasion IbeA of E.coli causing meningitis in which the surface probability and hydrophilicity were very similar to each other.It is concluded that the CglE fusion protein might be involved in the pathogenesis for E.coli causing meningitis to penetrate across the blood-brain barrier." @default.
- W2393625692 created "2016-06-24" @default.
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- W2393625692 date "2006-01-01" @default.
- W2393625692 modified "2023-09-23" @default.
- W2393625692 title "Cloning,expression and bioinformatics analysis for the novel gene cglE of Escherichia coli causing menengitis" @default.
- W2393625692 hasPublicationYear "2006" @default.
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