Matches in SemOpenAlex for { <https://semopenalex.org/work/W2393630877> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2393630877 abstract "Objective To construct expression recombinant plasmid containing the nuclear protein gene of human parainfluenza virus type 2 (HpI2) for the production of anti-parainfluenza vaccine. Method The gene for the nuclear protein (NP) of HpI2 was amplified by PCR from cell culture infected with HpI2. The PCR product was cloned into NdeⅠ and HindⅢ cut pQE2 vector to yield the recombinant plasmid pQE2-HpI2NP. The cloned fragment was sequenced and the fusion protein was expressed in E.coli BL21. Result The expressing recombinant plasmid pQE2-HpI2NP was constructed. The DNA sequence of the cloned HpI2NP gene was identical to the published sequence in GenBank. The fusion protein was expressed in E.coli and used to produce polyclonal antibodies. The specificity of the anti-NP antibodies was demonstrated by Western-blot. Conclusion The HpI2 NP was expressed in E.coli successfully and can be used to produce polyclonal antibodies." @default.
- W2393630877 created "2016-06-24" @default.
- W2393630877 creator A5047290878 @default.
- W2393630877 date "2008-01-01" @default.
- W2393630877 modified "2023-09-23" @default.
- W2393630877 title "Cloning and Expression and Polyclonal Antibodies Preparation for the Nucleocapsid Protein of Human Parainfluenza Virus Type 2" @default.
- W2393630877 hasPublicationYear "2008" @default.
- W2393630877 type Work @default.
- W2393630877 sameAs 2393630877 @default.
- W2393630877 citedByCount "0" @default.
- W2393630877 crossrefType "journal-article" @default.
- W2393630877 hasAuthorship W2393630877A5047290878 @default.
- W2393630877 hasConcept C104317684 @default.
- W2393630877 hasConcept C121050878 @default.
- W2393630877 hasConcept C123894998 @default.
- W2393630877 hasConcept C150194340 @default.
- W2393630877 hasConcept C153911025 @default.
- W2393630877 hasConcept C159047783 @default.
- W2393630877 hasConcept C159654299 @default.
- W2393630877 hasConcept C19924922 @default.
- W2393630877 hasConcept C199360897 @default.
- W2393630877 hasConcept C22744801 @default.
- W2393630877 hasConcept C2776415932 @default.
- W2393630877 hasConcept C33161422 @default.
- W2393630877 hasConcept C40767141 @default.
- W2393630877 hasConcept C41008148 @default.
- W2393630877 hasConcept C54355233 @default.
- W2393630877 hasConcept C86803240 @default.
- W2393630877 hasConcept C956191 @default.
- W2393630877 hasConceptScore W2393630877C104317684 @default.
- W2393630877 hasConceptScore W2393630877C121050878 @default.
- W2393630877 hasConceptScore W2393630877C123894998 @default.
- W2393630877 hasConceptScore W2393630877C150194340 @default.
- W2393630877 hasConceptScore W2393630877C153911025 @default.
- W2393630877 hasConceptScore W2393630877C159047783 @default.
- W2393630877 hasConceptScore W2393630877C159654299 @default.
- W2393630877 hasConceptScore W2393630877C19924922 @default.
- W2393630877 hasConceptScore W2393630877C199360897 @default.
- W2393630877 hasConceptScore W2393630877C22744801 @default.
- W2393630877 hasConceptScore W2393630877C2776415932 @default.
- W2393630877 hasConceptScore W2393630877C33161422 @default.
- W2393630877 hasConceptScore W2393630877C40767141 @default.
- W2393630877 hasConceptScore W2393630877C41008148 @default.
- W2393630877 hasConceptScore W2393630877C54355233 @default.
- W2393630877 hasConceptScore W2393630877C86803240 @default.
- W2393630877 hasConceptScore W2393630877C956191 @default.
- W2393630877 hasOpenAccess W2393630877 @default.
- W2393630877 hasRelatedWork W2038465288 @default.
- W2393630877 hasRelatedWork W2285615348 @default.
- W2393630877 hasRelatedWork W2289539600 @default.
- W2393630877 hasRelatedWork W2351853462 @default.
- W2393630877 hasRelatedWork W2365040639 @default.
- W2393630877 hasRelatedWork W2379582098 @default.
- W2393630877 hasRelatedWork W2379784772 @default.
- W2393630877 hasRelatedWork W2380927272 @default.
- W2393630877 hasRelatedWork W2387358509 @default.
- W2393630877 hasRelatedWork W2393864532 @default.
- W2393630877 hasRelatedWork W2397601145 @default.
- W2393630877 hasRelatedWork W2399188697 @default.
- W2393630877 hasRelatedWork W2399560651 @default.
- W2393630877 hasRelatedWork W2401148934 @default.
- W2393630877 hasRelatedWork W2414270712 @default.
- W2393630877 hasRelatedWork W2417088459 @default.
- W2393630877 hasRelatedWork W2977867073 @default.
- W2393630877 hasRelatedWork W3031829574 @default.
- W2393630877 hasRelatedWork W3101461218 @default.
- W2393630877 hasRelatedWork W2842947577 @default.
- W2393630877 isParatext "false" @default.
- W2393630877 isRetracted "false" @default.
- W2393630877 magId "2393630877" @default.
- W2393630877 workType "article" @default.