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- W2393646918 abstract "Objective To determine the cis-element in the regulation of rat ALAS-1 promoter by PGC-1β.MethodsTransient transfection with PGC-1β was done in Hep G2,then dual-luciferase reporter assay was performed to investigate fold activity of rat ALAS-1 promoter when over-expressing PGC-1β.Reconstructing ALAS-1 promoter reporters including a series of 5′-deletions and cis-element mutations,dual-luciferase reporter assay was performed to assay NRF-1 binding sites in rat ALAS-1 promoter.We constructed adenoviral PGC-1β and adenoviral siRNA of NRF1 and isolated rat primary hepatocytes and examined the effects of PGC-1β on the rat ALAS-1 transcription.Results Overexpression of PGC-1β stimulated the transcription of ALAS-1 in Hep G2 cells,while over-expressing NRF-1 alone had no stimulation effect.We constructed NRF-1 binding site mutated ALAS-1 promoter,the promoter activity was obviously diminished by overexpression of PGC-1β.PGC-1β promotes the transcription of ALAS-1 in the rat primary hepatocytes.When we used the siRNA to interfere the expression of NRF-1,the stimulating effect of PGC-1β on the ALAS-1 was attenuated.Conclusion PGC-1β promoting transcription of ALAS-1 is mainlyexplained by NRF-1 in Hep G2 cells and rat primary hepatocytes." @default.
- W2393646918 created "2016-06-24" @default.
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- W2393646918 date "2010-01-01" @default.
- W2393646918 modified "2023-09-26" @default.
- W2393646918 title "Investigation of the mechanism of PGC-1β regulating transcription of rat ALAS-1" @default.
- W2393646918 hasPublicationYear "2010" @default.
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