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- W2393652659 abstract "In order to study the effect of E.coli heat-labile enterotoxin LT(wild-type and mutant)proteins on the cytokine productions fluorescent quantitative PCR method for interferon(IFN-γ)detection was established and used for IFN-γ mRNA detection in mice immunized with LT or mutant recombinant LT protein intranasally.The results showed that the IFN-γ mRNA production in the mice immunized with wild-type and mutant LT proteins increased with time going,while the level of IFN-γmRNA in the mice of PBS control had not obvious variation.The IFN-γ mRNA of mice immunized with wild-type LT protein increased significantly and reached the highest level at 48 h,and then decreased at 60 h;The IFN-γ mRNA level of mice immunized with mutant LTRG protein got the highest at about 60 h,and then decreased at 72 h.From the above results it was concluded that both LT proteins could enhance transcriptional expression of IFN-γ in immunized mice and induce non-specific immune response against the antigen." @default.
- W2393652659 created "2016-06-24" @default.
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- W2393652659 date "2014-01-01" @default.
- W2393652659 modified "2023-09-23" @default.
- W2393652659 title "IFN-γ mRNA variation in mice spleens induced by LT protein" @default.
- W2393652659 hasPublicationYear "2014" @default.
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