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- W2393673225 abstract "Aim To establish five PCR-based reverse cross blot hybridization techniques for diagnosis of candida species.Methods The primer and specific oligonucleotide probes to conservative ERG11 gene of five common candida species were designed based on the gene fragments of PCR amplified products and hybond membrane was binded with the labeled probe for dot blot hybridization and coloration.Results The size of PCR amplified DNA fragment of five candida species was 350bp.The specificity and sensibility of hybond membrane reacted with PCR products of five common candida species were high.Conclusion The established PCR-based reverse cross blot hybridization methods allow exactly identify clinical common Candida species and wide application in clinical microorganism diagnosis." @default.
- W2393673225 created "2016-06-24" @default.
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- W2393673225 date "2011-01-01" @default.
- W2393673225 modified "2023-09-23" @default.
- W2393673225 title "The establishment of five reverse cross blot hybridization techniques for diagnosis of candida species" @default.
- W2393673225 hasPublicationYear "2011" @default.
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