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- W2393683247 abstract "Although complete sequence have not been reported, it has been known that polyalanine repeat domains are evident in the N terminal of spidroinⅠ that comprise the dragline silk of Nephila clavipes. 5 oligonucleotides were synthesized on the basis of published sequence data from cDNA clones for spidroinⅠ and used to amplification of the monomer sequence in corresponding to three polyalanine blocks. Multimer containing 2, 4 and 8-unit of monomer were obtained respectively using a head-to-tail construction strategy. Sequence analysis showed that the 8-unit multimer had 84% similarity to that of the MaSpⅠ, but the former one had a lower mRNA secondary structure energy. Then, these three kinds of multimer genes were cloned respectively into pET-30a (+), and induced with IPTG. Protein expression was characterized by the method of labeling and electrophoresis, since the addition of rifampin and 35S-Met together with IPTG blocks the expression of the Escherichia coli genome and only synthesis of the plasmid genes was detected. The result showed that special bands did exist in the cell lysate transformed with interest clone." @default.
- W2393683247 created "2016-06-24" @default.
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- W2393683247 date "2005-01-01" @default.
- W2393683247 modified "2023-09-23" @default.
- W2393683247 title "Synthesis of Spider Dragline Silk Genes and Their Expression in Escherichia coli" @default.
- W2393683247 hasPublicationYear "2005" @default.
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