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- W2393685736 abstract "Objective To study the roles of caspases in growth inhibition of HL-60 cells treated by ω-3 polyunsaturated fatty acids(ω-3 PUFA). Methods HL-60 cells were treated with 6.0×10 -5 mol/L eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA). The cellular proliferation capacity was tested with MTT assay and flow cytometry. The expression of caspase-3 and 8 was analyzed with RT-PCR and Western blotting. The activity of caspase-8 was analyzed with Ac-IETD-AMC, a caspase special substrate. To confirm the function of caspase activation in ω-3 PUFA induced apoptosis, Ac-IETD-CHO, an inhibitor of caspase, was used while cells were treated with ω-3 PUFA. Results In 2-3 d, the proliferation capacity of HL-60 cells in EPA or DHA groups was significantly decreased as compared with control group. HL-60 cells in 2 d after ω-3 PUFA treatment by flow cytometer showed cells increased in number at G_0/G_1, decreased at S and G_2/M phases, with an increasing apoptitic rate. The expression of caspase-3 and 8 and the activity of caspase-8 were increased in HL-60 cells treated with ω-3 PUFA as compared with control group, but the activity of caspase-8 in HL-60 cells decreased when cocultured with ω-3 PUFA and Ac-IETD-CHO for 2 d. Conclusion ω-3 PUFA could induce HL-60 cell apoptosis and inhibit the cell proliferation, in which the caspases network may play a key role." @default.
- W2393685736 created "2016-06-24" @default.
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- W2393685736 date "2005-01-01" @default.
- W2393685736 modified "2023-09-22" @default.
- W2393685736 title "Roles of caspases in growth inhibition of HL-60 cells treated by ω-3 polyunsaturated fatty acid" @default.
- W2393685736 hasPublicationYear "2005" @default.
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