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- W2393690043 abstract "cDNAs encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase(DXR),Secologanin synthase(SLS),Geraniol 10-hydroxylase(G10H) and Strictosidine synthase(STR) were obtained from the leaves of Catharanthus roseus with 2-step gateway PCR.These cDNA clones were inserted into the GATEWAY donor vector pDONR201 at the corresponding sites by BP clonase and followed by sequencing analysis,respectively.Thereafter,the entry clones were introduced into the GATEWAY destination vector pETG10A fused to 6xHIS-tag by LR clonase.Overexpression of recombinant fusion proteins DXR,SLS and STR were initiated after IPTG induction.These recombinant fusion proteins are now available for developing the antisera." @default.
- W2393690043 created "2016-06-24" @default.
- W2393690043 creator A5083425407 @default.
- W2393690043 date "2007-01-01" @default.
- W2393690043 modified "2023-09-25" @default.
- W2393690043 title "Cloning and Expression of cDNA Encoding Key Enzymes(DXR,SLS,G10H and STR) in Terpene Indole Alkaloids Biosynthesis Pathway from Catharanthus roseus" @default.
- W2393690043 hasPublicationYear "2007" @default.
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