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- W2393706508 abstract "The full mRNA of kenaf Ms5 gene was tried to clone with RT-PCR and 3′RACE methods based on the known partial 5′-end cDNA sequence.The sequencing results showed that two transcripted cDNAs of Ms5 gene were cloned from the anther of kenaf.The length of two transcripts named as HcMs5-α and HcMs5-β were 972 bp and 1 105 bp,respectively.The structure analysis showed that both of them contained an 858 bp ORF,encoding a protein of 285 amino acids with predicted molecular weight of 32.4 ku and a isoelectric point of 7.62.The sequencing results showed that 5′-end 972 bp sequence of the two transcripts was identical,but HcMs5-β possessed an additional 133 bp sequence at 3′-end after 972 bp compared with HcMs5-α.The alternative termination of transcription in the 3′UTR of kenaf Ms5 maybe related with the post transcriptional regulation of the gene.It is the first report about the multiple transcripts of Ms5 gene in higher plant." @default.
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- W2393706508 date "2012-01-01" @default.
- W2393706508 modified "2023-09-23" @default.
- W2393706508 title "Cloning and multiple transcript analysis of Ms5 gene in kenaf(Hibiscus canabius L.)" @default.
- W2393706508 hasPublicationYear "2012" @default.
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