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- W2393719783 abstract "The NS1 gene of H3N2 subtype swine influenza virus was expressed in prokaryotic expression system to obtain recombinant protein which was used to develop an effective diagnostic method.The NS1 gene was amplified by reverse transcriptase polymerase chain reaction(RT-PCR) and cloned into a prokaryotic expression vector,pET- 32a(+),and then expressed in E.coil Rosetta~(IM) after induced by 0.5 mmol/L Isopropyl β-D-thiogalactoside (IPTG).The recombinant protein was purified and identified its antigencity by western-blotting.Based on the western -blotting test,an indirect enzyme-linked immunosorbent assay(ELISA) was constructed to develop a H3N2 subtype swine influenza virus detection kit.The recombinant protein was about 43 500.The result of Western-blotting indicated that the recombinant had specifically immunologic reactionogenicity with postive serum.The result of ELISA showed that it has good specificity,sensitivity and reproducibility without cross reaction to some kinds of common infection disease pathogeny in pigs and was prospective to develop an efficient detection kit for to detection of SIV in clinic." @default.
- W2393719783 created "2016-06-24" @default.
- W2393719783 creator A5083397947 @default.
- W2393719783 date "2011-01-01" @default.
- W2393719783 modified "2023-09-23" @default.
- W2393719783 title "Cloning and expression of swine influenza virus subtype H3N2 and establishment of an indirect ELISA" @default.
- W2393719783 hasPublicationYear "2011" @default.
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