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- W2393752023 abstract "Agrobacterium tumefaciens mediated transformation system of Cynodon dactylon (L.) Pers. wasstudied by using GUS gene transient expression method. Mature seed derived callus from C. dactylon wastransformed by GUS gene containing A. tumefaciens LBA4404/pCAMBIA1301. Frequency of the transforma-tion was detected, and the optimal conditions for C. dactylon genetic transformation were determined. In thissystem, it was found that the optimal conditions to get higher transformation efficiency were co-cultivationwith OD600 0.5–0.8 A. tumefaciens solution for 2 d under dark after pre-culture for 10 d. The transformationfrequency was significantly improved by adding 100 mmol·L-1 acetosyingone into co-cultivation medium. Add-ing A. tumefaciens solution on surface of filter paper was found to be a better infecting method of A. tumefaciensrather than suspending in A. tumefaciens solution. The transformation efficiency in dark condition to cocultivationwas higher than that of 12/12-h photoperiod. Under these optimal conditions, the rate of transient expression ofGUS gene in C. dactylon was 36.36%." @default.
- W2393752023 created "2016-06-24" @default.
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- W2393752023 date "2005-01-01" @default.
- W2393752023 modified "2023-09-23" @default.
- W2393752023 title "Factors Influencing Agrobacterium-mediated Transformation of Cynodon dactylon (L.) Pers." @default.
- W2393752023 hasPublicationYear "2005" @default.
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