Matches in SemOpenAlex for { <https://semopenalex.org/work/W2393805536> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2393805536 abstract "Objective To investigate the effect of endothelial-monocyte activating polypeptide Ⅱ(EMAP-Ⅱ) on the distribution and interaction of the tight junction associated proteins occludin and ZO-1 as well as the change of the activity of protein kinase C(PKC),and further analyze the action of PKC in the process of EMAP-Ⅱ opening the tight junction.Methods The blood-tumor barrier(BTB) models were established by extracting and culturing the rat brain microvascular endothelial cells(BMEC).Subjects were divided into 7 groups:model group,EMAP-Ⅱ 0.5 h group,EMAP-Ⅱ 1 h group,EMAP-Ⅱ 2 h group,EMAP-Ⅱ 3 h group,EMAP-Ⅱ 6 h group and EMAP-Ⅱ 12 h group.The Milicell-ERS system was used to measure the transendothelial electrical resistance(TEER).The distribution and location of occludin and ZO-1 were detected by double-immunofluorescence assay.Immunoprecipitation assay was used to test the interaction of occludin and ZO-1 in BMEC.The activity of total PKC was detected by PKC assay kit.Results In contrast to model group,EMAP-Ⅱ groups showed that EMAP-Ⅱ obviously decreased the TEER of in BTB model in vitro and increased its permeability.In model group,the tight junction associated proteins occludin and ZO-1 was localized to the cell boundaries with continuous distribution and both of them presented co-location.After EMAP-Ⅱ administering,occludin and ZO-1 were discontinuously distributed in the cellular boundaries and the co-location of them was attenuated.The result of immunoprecipitation showed that EMAP-Ⅱ could attenuate the combination of occludin and ZO-1.The above actions were most obvious when EMAP-Ⅱhad been administered for 1h(P 0.01).Meanwhile,the activity of total PKC was increased significantly after EMAP-Ⅱ administering and the peak turned up at EMAP-Ⅱ action 1 h(P 0.01).Conclusion EMAP-Ⅱ could regulate the interaction of tight junction associated proteins occludin and ZO-1 in BMEC and open the tight junction.PKC might be one of the mechanisms of EMAP-Ⅱ regulating the interaction of occludin and ZO-1 and opening the tight junction." @default.
- W2393805536 created "2016-06-24" @default.
- W2393805536 creator A5064488623 @default.
- W2393805536 date "2012-01-01" @default.
- W2393805536 modified "2023-09-26" @default.
- W2393805536 title "Regulation and Mechanism of EMAP-II on the Interaction of Tight Junction Associated Protein Occludin and ZO-1" @default.
- W2393805536 hasPublicationYear "2012" @default.
- W2393805536 type Work @default.
- W2393805536 sameAs 2393805536 @default.
- W2393805536 citedByCount "0" @default.
- W2393805536 crossrefType "journal-article" @default.
- W2393805536 hasAuthorship W2393805536A5064488623 @default.
- W2393805536 hasConcept C159654299 @default.
- W2393805536 hasConcept C177779419 @default.
- W2393805536 hasConcept C185592680 @default.
- W2393805536 hasConcept C195794163 @default.
- W2393805536 hasConcept C202751555 @default.
- W2393805536 hasConcept C203014093 @default.
- W2393805536 hasConcept C2775962179 @default.
- W2393805536 hasConcept C55493867 @default.
- W2393805536 hasConcept C62478195 @default.
- W2393805536 hasConcept C71829478 @default.
- W2393805536 hasConcept C86803240 @default.
- W2393805536 hasConcept C95444343 @default.
- W2393805536 hasConceptScore W2393805536C159654299 @default.
- W2393805536 hasConceptScore W2393805536C177779419 @default.
- W2393805536 hasConceptScore W2393805536C185592680 @default.
- W2393805536 hasConceptScore W2393805536C195794163 @default.
- W2393805536 hasConceptScore W2393805536C202751555 @default.
- W2393805536 hasConceptScore W2393805536C203014093 @default.
- W2393805536 hasConceptScore W2393805536C2775962179 @default.
- W2393805536 hasConceptScore W2393805536C55493867 @default.
- W2393805536 hasConceptScore W2393805536C62478195 @default.
- W2393805536 hasConceptScore W2393805536C71829478 @default.
- W2393805536 hasConceptScore W2393805536C86803240 @default.
- W2393805536 hasConceptScore W2393805536C95444343 @default.
- W2393805536 hasLocation W23938055361 @default.
- W2393805536 hasOpenAccess W2393805536 @default.
- W2393805536 hasPrimaryLocation W23938055361 @default.
- W2393805536 hasRelatedWork W1128055432 @default.
- W2393805536 hasRelatedWork W1571406711 @default.
- W2393805536 hasRelatedWork W1965836316 @default.
- W2393805536 hasRelatedWork W1989980183 @default.
- W2393805536 hasRelatedWork W2012114568 @default.
- W2393805536 hasRelatedWork W2018778283 @default.
- W2393805536 hasRelatedWork W2038363941 @default.
- W2393805536 hasRelatedWork W2039967795 @default.
- W2393805536 hasRelatedWork W2052495642 @default.
- W2393805536 hasRelatedWork W2063638556 @default.
- W2393805536 hasRelatedWork W2073118455 @default.
- W2393805536 hasRelatedWork W2122692132 @default.
- W2393805536 hasRelatedWork W2143453317 @default.
- W2393805536 hasRelatedWork W2146711122 @default.
- W2393805536 hasRelatedWork W2148504803 @default.
- W2393805536 hasRelatedWork W2169584339 @default.
- W2393805536 hasRelatedWork W2367262016 @default.
- W2393805536 hasRelatedWork W2769740891 @default.
- W2393805536 hasRelatedWork W2977348419 @default.
- W2393805536 hasRelatedWork W89214381 @default.
- W2393805536 isParatext "false" @default.
- W2393805536 isRetracted "false" @default.
- W2393805536 magId "2393805536" @default.
- W2393805536 workType "article" @default.