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- W2393839805 abstract "Objective: To investigate the effect of seawater(SW) immersion on the apoptosis and proliferation of epidermic keratinocytes in hairless mice skin.Methods: Twenty-four healthy male hairless mice were randomly assigned to 4 groups.the light microscope,TdT-mediated dUTP nick end labeling(TUNEL),and immunohistochemistry of Ki-67 and Cyclin D1 were used to study the apoptosis and proliferation characteristics of epidermic cells in the hairless mice skin following SW immersion.Results: Keratinocytes of both the basal and granulosum stratum showed apoptosis after SW immersion for 3 h,presenting cell shrinkage,condensation of nuclear chromatin,eosinophilic cytoplasm,and karyorrhexis or nuclear karyolysis,while in the control group,only low apoptosis was found by TUNEL in the basal stratum,and the apoptosis increased in the 6 h and 12 h SW immersion groups(P0.01).The rate of Ki-67 positive cells increased slightly in the 3 h and 6 h SW immersion groups (P0.05),but significantly in the 12 h(P 0.01) group.Cyclin D1 expression was significantly up-regulated in the 6 h and 12 h groups(P0.05,P0.01).All the changes were positively correlated with the immersion time.Conclusion:Prolonged SW immersion can induce the apoptosis and proliferation of keratinocytes,both involved in the pathophysiological process." @default.
- W2393839805 created "2016-06-24" @default.
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- W2393839805 date "2007-01-01" @default.
- W2393839805 modified "2023-09-23" @default.
- W2393839805 title "Seawater immersion induces apoptosis and proliferation of epidermic cells" @default.
- W2393839805 hasPublicationYear "2007" @default.
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