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- W2393847095 abstract "Through genetic engineering fermentation in E.Coli β-mannanase.Though β-manganase producing strain of Aspergillums niger as the material,Aspergillums niger obtained the cDNA of MAN1 fragment by RT-PCR amplification of β-manganase gene.The fragment was connected with PMD18-T and sequenced.Blast comparisons results showed the sequence number XM001400053 of GenBank have 100% similarity.PMD18-MAN1 and PET-32b were double ligated to construct the prokaryotic expression vector PET-MAN1.When restriction endonuclease analysis is correct,the vector was introduced into E.coli BL21.Recombinant strains are induced by IPTG,the expressed products were SDS-PAGE and though DNS method in different temperatures and different pH determinate the mannan fusion protein activity.As the results: After IPTG induction,transformed by the fusion protein about 62 ku,it shows that the optimum time was 6 h,fusion proteins form soluble fractions.it shows that the optimum temperature was 55 ℃,the optimum pH of 5.5,in this condition,mannanase activity was 48 IU·mL-1.Results to the industrial production of β-mannanase to explore new ways." @default.
- W2393847095 created "2016-06-24" @default.
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- W2393847095 date "2011-01-01" @default.
- W2393847095 modified "2023-09-28" @default.
- W2393847095 title "Expression of Aspergillus MAN1 gene in E.coli and its enzymatic characterization analysis" @default.
- W2393847095 hasPublicationYear "2011" @default.
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