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- W2393853883 abstract "Objective:To establish an effective Tricine-SDS-PAGE system to separate cyclic lipopeptide with 1kDa molecular weight.Methods: Based on the characteristics of cyclic lipopeptide,the composition and proportion of gels,the speed of polymerization and some other conditions were optimized.Results: After adjusted the proportion of the gels,the stacking gel was 7%T and 3%C,the spacer gel was 12%T and 3%C and the separating gel was 16.5%T and 6%C.Then urea and glycerol were added to gel and the speed of the gel polymerization was raised.The band of electrophoresis was clear、balanced and compact.The molecular weight of the cyclic lipopeptide was 1056Da which was accordance to that of MS.Conclusions: The new Tricine-SDS-PAGE is an effective method to separate cyclic lipopeptide with 1kDa molecular weight." @default.
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- W2393853883 date "2005-01-01" @default.
- W2393853883 modified "2023-09-26" @default.
- W2393853883 title "An Effective Electrophoresis Method to Separate Cyclic Lipopeptide with 1kDa Molecular Weight" @default.
- W2393853883 hasPublicationYear "2005" @default.
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