Matches in SemOpenAlex for { <https://semopenalex.org/work/W2393855712> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2393855712 endingPage "5" @default.
- W2393855712 startingPage "672" @default.
- W2393855712 abstract "Using monoclonal antibody (McAb) technique to analyze polypeptide antigens of Mycobacterium strains and try to find out the best method for purification of McAb.McAbs against H37Rv strain were produced by routine procedure. McAb-producing hybridomas were detected by ELISA and immunoblots technique. 24 strains of mycobacterium were grown on Loewenstein-Jensen medium for 1 to 3 weeks at 37 degrees C. H37Rv strain were grown on Loewenstein-Jensen medium or Sauton liquid medium. Ammonium sulfate, caprylic acid precipitation and ion exchange chromatography methods were used to purify McAbs against antigens of H37Rv strain.14 McAb-produing hybridomas were obtained. C2 McAb reacted with all 24 strains of mycobacterium and 7C12 only reacted with H37Rv, H37Ra, M. bovis, BCG strains, C2 and 7C12 reacted with secreted proteins of H37Rv strain. 40,000/38,000 antigen of H37Rv strain was expressed differently in Loewenstein-Jensen medium and Sauton liquid medium. Relative activity of all McAbs was between 10(-4)-10(-7). The results of purified McAb showed that ammonium sulfate precipitation method could have the highest rate of recovery. Ion exchange chromatography method could have the highest purity. Caprylic acid method can remove albumin effectively and the purification of McAbs in ascific fluid was simple and easy to perform.Polypeptide antigens of H37Rv against by C2, 7C12 McAb were the secreted proteins. For purification of McAb, the results suggested that ion exchange chromatography method can be applied for analysis and caprylic acid method for a very large volume of ascitic fluid." @default.
- W2393855712 created "2016-06-24" @default.
- W2393855712 creator A5028297194 @default.
- W2393855712 creator A5034298091 @default.
- W2393855712 creator A5042380331 @default.
- W2393855712 date "2000-11-01" @default.
- W2393855712 modified "2023-09-23" @default.
- W2393855712 title "[Production, characterization and purification of monoclonal antibodies against antigens of Mycobacterium tuberculosis H37Rv strain]." @default.
- W2393855712 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11225032" @default.
- W2393855712 hasPublicationYear "2000" @default.
- W2393855712 type Work @default.
- W2393855712 sameAs 2393855712 @default.
- W2393855712 citedByCount "0" @default.
- W2393855712 crossrefType "journal-article" @default.
- W2393855712 hasAuthorship W2393855712A5028297194 @default.
- W2393855712 hasAuthorship W2393855712A5034298091 @default.
- W2393855712 hasAuthorship W2393855712A5042380331 @default.
- W2393855712 hasConcept C105702510 @default.
- W2393855712 hasConcept C147483822 @default.
- W2393855712 hasConcept C153911025 @default.
- W2393855712 hasConcept C159654299 @default.
- W2393855712 hasConcept C181199279 @default.
- W2393855712 hasConcept C185592680 @default.
- W2393855712 hasConcept C193363816 @default.
- W2393855712 hasConcept C203014093 @default.
- W2393855712 hasConcept C206967267 @default.
- W2393855712 hasConcept C2776428949 @default.
- W2393855712 hasConcept C2778022156 @default.
- W2393855712 hasConcept C2778397350 @default.
- W2393855712 hasConcept C2780374374 @default.
- W2393855712 hasConcept C2780925461 @default.
- W2393855712 hasConcept C43617362 @default.
- W2393855712 hasConcept C523546767 @default.
- W2393855712 hasConcept C542903549 @default.
- W2393855712 hasConcept C543025807 @default.
- W2393855712 hasConcept C54355233 @default.
- W2393855712 hasConcept C55493867 @default.
- W2393855712 hasConcept C86803240 @default.
- W2393855712 hasConceptScore W2393855712C105702510 @default.
- W2393855712 hasConceptScore W2393855712C147483822 @default.
- W2393855712 hasConceptScore W2393855712C153911025 @default.
- W2393855712 hasConceptScore W2393855712C159654299 @default.
- W2393855712 hasConceptScore W2393855712C181199279 @default.
- W2393855712 hasConceptScore W2393855712C185592680 @default.
- W2393855712 hasConceptScore W2393855712C193363816 @default.
- W2393855712 hasConceptScore W2393855712C203014093 @default.
- W2393855712 hasConceptScore W2393855712C206967267 @default.
- W2393855712 hasConceptScore W2393855712C2776428949 @default.
- W2393855712 hasConceptScore W2393855712C2778022156 @default.
- W2393855712 hasConceptScore W2393855712C2778397350 @default.
- W2393855712 hasConceptScore W2393855712C2780374374 @default.
- W2393855712 hasConceptScore W2393855712C2780925461 @default.
- W2393855712 hasConceptScore W2393855712C43617362 @default.
- W2393855712 hasConceptScore W2393855712C523546767 @default.
- W2393855712 hasConceptScore W2393855712C542903549 @default.
- W2393855712 hasConceptScore W2393855712C543025807 @default.
- W2393855712 hasConceptScore W2393855712C54355233 @default.
- W2393855712 hasConceptScore W2393855712C55493867 @default.
- W2393855712 hasConceptScore W2393855712C86803240 @default.
- W2393855712 hasIssue "11" @default.
- W2393855712 hasLocation W23938557121 @default.
- W2393855712 hasOpenAccess W2393855712 @default.
- W2393855712 hasPrimaryLocation W23938557121 @default.
- W2393855712 hasRelatedWork W1543286979 @default.
- W2393855712 hasRelatedWork W1992240357 @default.
- W2393855712 hasRelatedWork W2026610848 @default.
- W2393855712 hasRelatedWork W2098600028 @default.
- W2393855712 hasRelatedWork W2352683277 @default.
- W2393855712 hasRelatedWork W2359976329 @default.
- W2393855712 hasRelatedWork W2368877937 @default.
- W2393855712 hasRelatedWork W2370849782 @default.
- W2393855712 hasRelatedWork W2393855712 @default.
- W2393855712 hasRelatedWork W2466573242 @default.
- W2393855712 hasVolume "23" @default.
- W2393855712 isParatext "false" @default.
- W2393855712 isRetracted "false" @default.
- W2393855712 magId "2393855712" @default.
- W2393855712 workType "article" @default.