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- W2393872289 abstract "To investigate the effect of low salinity stress on gene expression of hepatopancreas in shrimp,we applied suppression subtractive hybridization(SSH) in juvenile white shrimp(Litopenaeus vannamei) exposed to low salinity.The shrimps(initial body weight,0.27±0.01 g) cultured at salinity 2 and salinity 30 for 56 days respectively,forward and reverse subtractive cDNA libraries of hepatopancreas in shrimp cultured at these two salinities were constructed.Subtraction efficiencies were about 25 and 25～10-fold enrichment in hepatopancreas of forward and reverse cDNA libraries.A total of 200(80 from forward,120 from reverse) randomly selected clones was sequenced and acquired 179 ESTs with more than 100 nucleotides.19 contigs and 54 singletons were generated from a total of 73 Unigenes after CAP3 online sequence assembly program.Results of bioinformatics analysis indicated that the unigenes encoded proteins corresponded to a wide range of functions including immune-related proteins and enzymes,metabolism-related enzymes,ribosomal proteins,apoptosis-related proteins and transfer proteins,which illuminated that the corresponding physiology response induced by low salinity was very complicated.The cDNA subtractive library could well reflect the gene information of L.vannamei induced by long-term low salinity-stress,results of which provide the basic data to research of relationship between environmental stress and gene expression of liver." @default.
- W2393872289 created "2016-06-24" @default.
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- W2393872289 date "2012-01-01" @default.
- W2393872289 modified "2023-09-24" @default.
- W2393872289 title "Construction of the Hepatopancreas Subtractive cDNA Library of Litopenaeus vannamei Induced by Hyposmotic Stress and ESTs Analysis" @default.
- W2393872289 hasPublicationYear "2012" @default.
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