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- W2393886002 abstract "Pseudomonas syringae pv.mori is a pathogen of the buds and tender stems of mulberry tree and causes rupture.However,the contributing mechanism is unknown.The specific primers were designed to amplify the putative cellulase gene of P.syringae pv.mori according to the sequence from Pseudomonas syringae pv.syringae B728a in GenBank(C75).The PCR product was cloned into the vector pET28a and sequenced.It showed that the open reading frame of the gene has 1 173 base pairs long,and encode 390 amino acids.The similarity is 94 percent between the genes and 97 percent between the proteins from P.syringae pv.mori and P.syringae pv.syringae B728a,respectively.The functional motif and conservative sites of this peptide were estimated by online analysis.The gene was expressed in the E.coli strain DE3 by the inducer IPTG.The result showed that the cellulase gene could be highly expressed by SDS-PAGE and Western Blotting.The cellulase activity of the gene was detected as 2.3×103 U/L in the bacteria lysate.It indicated that the gene had cellulase activity." @default.
- W2393886002 created "2016-06-24" @default.
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- W2393886002 date "2007-01-01" @default.
- W2393886002 modified "2023-09-25" @default.
- W2393886002 title "Cloning of Putative Cellulase Gene of Pseudomonas syringae pv.mori and its Expression Analysis" @default.
- W2393886002 hasPublicationYear "2007" @default.
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