Matches in SemOpenAlex for { <https://semopenalex.org/work/W2393897229> ?p ?o ?g. }
Showing items 1 to 55 of
55
with 100 items per page.
- W2393897229 abstract "Aim To prepare and characterize the monoclonal antibody against poly-mannaguluronic acid (911). Methods A hybridoma cell line was obtained by cloning for 3 times those cells that secret antibody against 911 after the fusion of NS-1 myelome cells with spleen cells from Balb/c mice immunized with a 911-bovine serum albumin conjugate, prepared by reductive amination. Hybridoma was inoculated to Balb/c mouse to induce ascites. The antibody was purified by ammonium sulfate and Protein A - Sepharose CL-4B. DD3 was confirmed to be fusion cells after determination of DNA content with flow cytometry. Competitive inhibitory test and Biacore confirmed the cross-reactivity of the antibody with other endogenous polysaccharides or with alginic acid sodium. Igs′ classes and subclasses were identified by Isostrip TM method. The affinity of DD3 was verified by ELISA. Results A hybridoma cell line secreting monoclonal antibody against 911 (marine sulfate polysaccharide) named DD3 was obtained. The DD3 ascites contained specific antibody with titer over 1.0×10 5. There was no cross-reactivity of these antibodies with other endogenous polysaccharides or with alginic acid sodium. The immunoglobulin subclass of DD3 was IgG2a, κ type. The affinity of DD3 was 2.0×10 8 L·mol -1 . Conclusion One hybirdoma line (DD3) secreting monoclonal antibody against 911 was established to provide a potential method for the pharmacokinetic study of 911." @default.
- W2393897229 created "2016-06-24" @default.
- W2393897229 creator A5015296795 @default.
- W2393897229 date "2003-01-01" @default.
- W2393897229 modified "2023-09-24" @default.
- W2393897229 title "Preparation and characterization of monoclonal antibody against marine sulfated polysaccharide drug poly-mannaguluronic acid" @default.
- W2393897229 hasPublicationYear "2003" @default.
- W2393897229 type Work @default.
- W2393897229 sameAs 2393897229 @default.
- W2393897229 citedByCount "0" @default.
- W2393897229 crossrefType "journal-article" @default.
- W2393897229 hasAuthorship W2393897229A5015296795 @default.
- W2393897229 hasConcept C153911025 @default.
- W2393897229 hasConcept C159654299 @default.
- W2393897229 hasConcept C185592680 @default.
- W2393897229 hasConcept C203014093 @default.
- W2393897229 hasConcept C542903549 @default.
- W2393897229 hasConcept C55493867 @default.
- W2393897229 hasConcept C86803240 @default.
- W2393897229 hasConcept C86807702 @default.
- W2393897229 hasConceptScore W2393897229C153911025 @default.
- W2393897229 hasConceptScore W2393897229C159654299 @default.
- W2393897229 hasConceptScore W2393897229C185592680 @default.
- W2393897229 hasConceptScore W2393897229C203014093 @default.
- W2393897229 hasConceptScore W2393897229C542903549 @default.
- W2393897229 hasConceptScore W2393897229C55493867 @default.
- W2393897229 hasConceptScore W2393897229C86803240 @default.
- W2393897229 hasConceptScore W2393897229C86807702 @default.
- W2393897229 hasLocation W23938972291 @default.
- W2393897229 hasOpenAccess W2393897229 @default.
- W2393897229 hasPrimaryLocation W23938972291 @default.
- W2393897229 hasRelatedWork W1533355477 @default.
- W2393897229 hasRelatedWork W173655547 @default.
- W2393897229 hasRelatedWork W1864792619 @default.
- W2393897229 hasRelatedWork W2014760954 @default.
- W2393897229 hasRelatedWork W2039266676 @default.
- W2393897229 hasRelatedWork W2094639405 @default.
- W2393897229 hasRelatedWork W2318766225 @default.
- W2393897229 hasRelatedWork W2349618746 @default.
- W2393897229 hasRelatedWork W2360489324 @default.
- W2393897229 hasRelatedWork W2366761756 @default.
- W2393897229 hasRelatedWork W2371121527 @default.
- W2393897229 hasRelatedWork W2375668706 @default.
- W2393897229 hasRelatedWork W2375983643 @default.
- W2393897229 hasRelatedWork W2386155408 @default.
- W2393897229 hasRelatedWork W2388873006 @default.
- W2393897229 hasRelatedWork W2388899694 @default.
- W2393897229 hasRelatedWork W2390265136 @default.
- W2393897229 hasRelatedWork W2393162208 @default.
- W2393897229 hasRelatedWork W2413402096 @default.
- W2393897229 hasRelatedWork W2978251081 @default.
- W2393897229 isParatext "false" @default.
- W2393897229 isRetracted "false" @default.
- W2393897229 magId "2393897229" @default.
- W2393897229 workType "article" @default.