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- W2393972271 abstract "Objective To observe the influence of ozone on the ultrastructures of nucleus pulposus. Methods Two canines were subjected to the injection of ozone oxygen gas (3ml with concentration of 30μg/ml) into the L4/5 and L5/6 discs respectively with 21G Chiba needle. The procedure was repeated a week later . The animals were sacrificed and sampled for the observation of ultrastructure under electron microscopy one month and two months after the procedure respectively. Results The nucleus pulposuses looked like trans parent jelly in normal discs shrunk apparently and presented as white cheese like, unstretchable and friable structure after O 3 O 2 injection. Under electron microscopy, living cells in the experimental nucleus pulposuses were sparse, and dead cells could often be seen. Differing from normal nucleus pulposus cells with long dendrites, the cells in the experimental nucleus pulposuses showed no dendrites or only short and small ones. The amount of cell organelles and glycogen granules reduced apparently. In the stroma, no normol great bullous structures could be observed, and the collagen fibers turned into dense matrix from net like matrix in normal state. Conclusions Ozone can damage the function of cell and tissue structures causing water lose and volume reduction in the neucleus pulposus." @default.
- W2393972271 created "2016-06-24" @default.
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- W2393972271 date "2001-01-01" @default.
- W2393972271 modified "2023-09-24" @default.
- W2393972271 title "Influence of ozone on the ultrastructures of nucleus pulposus" @default.
- W2393972271 hasPublicationYear "2001" @default.
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