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- W2394007071 abstract "Objective To evaluate the effects of CO2-independent culture cell system on the proliferation of prostate carcinoma cell line PC-3.Methods Prostate carcinoma cell line PC-3 was cultured using CO2-independent culture cell system and CO2-dependent culture cell system at 37 ℃ in 50 mL/L carbon dioxide incubator and at 37 ℃ in air incubator.The pH of culture medium with the two culture cell systems was detected.Differences in cell viability were assayed with CCK-8 test and colony forming assay.The expressions of survivin and caspase-3 mRNA of cell culturing with the two systems were detected by RT-PCR assay.Results Under the two culture cell systems,the pH trend was the same(P0.05),PC-3 cell proliferation and the expressions of survivin and caspase-3 mRNA of the cell had no obvious differences(P0.05).Conclusion CO2-independent culture cell system is parallel to CO2-dependent culture cell system in their effects on cell proliferation and gene expression." @default.
- W2394007071 created "2016-06-24" @default.
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- W2394007071 date "2010-01-01" @default.
- W2394007071 modified "2023-09-24" @default.
- W2394007071 title "Effects of CO_2-independent cell culture system on the proliferation of prostate carcinoma cell line PC-3" @default.
- W2394007071 hasPublicationYear "2010" @default.
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