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- W2394016687 abstract "To detect multiplex single nucleotide polymorphisms(SNPs)simultaneously,a new method was established by combining ALM-ASA with microfabricated CE-chip.Taking the CYP2D6 gene as an example,six SNPs,100CT(P34S),1707Tdel(frameshift),1758GT(stop codon),2470TC,2549Adel(frameshift)and 2613AGAdel(K281del),were typed by four steps consisting of preamplification,digestion and ligation,allele-specific amplification,and amplicon separation by chip-CE.The genotyping results of 20 different genome samples by 6-plexed ALM-ASA were completely consistent with those obtained by polymerase chain reaction-restriction fragment length polymorphism analysis(PCR-RFLP),indicating that the multiplex approach established in this study was accurate and inexpensive.As the small reagent consumption by CE-chip device,a low cost for SNP typing was achieved together with the multiplex PCR technology proposed in this report.Neither modification of microchip channels nor clean-up process of PCR products was required;this greatly shortens the whole time for SNP typing." @default.
- W2394016687 created "2016-06-24" @default.
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- W2394016687 date "2006-01-01" @default.
- W2394016687 modified "2023-09-23" @default.
- W2394016687 title "Simultaneous Detection of Multiplex SNPs by Adapter-ligation Mediated Allele-specific Amplification(ALM-ASA)on CE-chip Electrophoresis Device" @default.
- W2394016687 hasPublicationYear "2006" @default.
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