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- W2394020443 abstract "Objective: To establish a simple and credible culture system of rat cerebral microvascular endothelial cells(CMECs). Methods:Cerebral microvascular endothelial cells isolated from neogenetic SD rats were primary cultured and subcultured by homogenate, filtration, collagenase digestion and differential conglutination.Results:The cultured cells displayed monolayer growth and looked like typical cobblestones at about 7～9 th day. They were identified as endothelial cells by morphological observation and immunocytochemistry studies of factor Ⅷ relative antigen.Conclusion: This culture system would provide a useful tool for studying diseases related to cerebral vessels in vitro." @default.
- W2394020443 created "2016-06-24" @default.
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- W2394020443 date "2004-01-01" @default.
- W2394020443 modified "2023-09-26" @default.
- W2394020443 title "Cultivation of rat brain microvascular endothelial cells in vitro" @default.
- W2394020443 hasPublicationYear "2004" @default.
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