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- W2394042387 abstract "Objective To set up the theories of uncertainty and reliability(U R) in average method(AM) of parallel multiple wavelength(PMW) chromatographic fingerprints(PMW-FP-AM) of traditional Chinese medicine and evaluate the U R of the corresponding referential fingerprints(RFP) and each batch of Ermiao Wan(EMW) detected under the parallel six wavelengths for high performance liquid chromatography fingerprints(PSW-HPLC-FP) by AM. Methods The chromatographic fingerprints were determined by reversed-phase HPLC under PSW,in which the AM and systematic quantified fingerprint method(SQFM) were used to comprehensively evaluate the quality of 11 batches of EMWs. Results A total of 53(254 nm),54(265 nm),51(284 nm),57(305 nm),53(326 nm),and 47(340 nm) common peaks were respectively marked with berberine peak as the referential peak and EMW-PSW-FPs were established.As the same time S2,S6,and S10(gradeⅠ)were identified as having the best quality;S7,S8,and S9(gradeⅡ)with better quality;S5 and S11(grade Ⅲ)with good quality;S1,S3 and S4(gradeⅥ)common quality by PSW-FP-AM.The reliabilities(all over 0.97) of EMW-PSW-FP-AM corresponding norm were reliable,which showed the information of fingerprint quantity and distribution proportion,and the overall contents reflected quality by RFP were highly reliable.The reliability(0.918-0.984)in qualitative and quantitative information for S2, S5,S6,S7,S8,S9,and S10 was classified as very high,that of S11 was reliable,while that(0.778-0.881)of S1,S3 and S4 was moderate. Conclusion The evaluation method of uncertainties in PMW-FP-AM can accurately reflect the variations in the quality of traditional Chinese medicine,and those of reliabilities would exactly calculate the degrees of reliabilities of qualitative and quantitative information included in the RFP and each sample fingerprint by PMW-FP-AM." @default.
- W2394042387 created "2016-06-24" @default.
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- W2394042387 date "2011-01-01" @default.
- W2394042387 modified "2023-09-23" @default.
- W2394042387 title "Uncertainty and reliability of average method in assessing the parallel multiple wavelength chromatographic fingerprint of traditional Chinese medicine" @default.
- W2394042387 hasPublicationYear "2011" @default.
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