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- W2394055027 abstract "In order to develop drought-resistant varieties of cabbage,taking Chinese cabbage Erbaotou as test material and cotyledon petioles as transformation receptor,the establishment of genetic transformation system of Chinese cabbage drought-resistant were studied by Agrobacterium-mediated.The results showed that seed disinfection method was soaking with 75%ethanol for 30sfirst;and then soaking with 0.1% mercuric chloride for 10minutes,the budding rate could be higher and sterile.The best differential medium was MS+6-BA 2.5mg·L-1+NAA 0.6mg·L-1,differentiation rate of adventitious bud was 30%.The best rooting medium was MS+NAA 0.4mg·L-1,the roots were thick and strong.Leaf disc transformation method was used to establish genetic transformation of Chinese cabbage by Agrobacterium-mediated,which transfered T-DNA containing drought-resistant gene T59into explants,resistance seedlings were acquired by Hyg screening.Effect of several factors on Agrobacterium-mediated transformation was studied through transgenic test,the following conclusions could be reached:the best Agrobacteriumdilution was 8and 16times,the best common cultivation time was 2d,the optimal antibiotic combination was 400mg·L-1 Cb+200mg·L-1 Cef." @default.
- W2394055027 created "2016-06-24" @default.
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- W2394055027 date "2013-01-01" @default.
- W2394055027 modified "2023-09-24" @default.
- W2394055027 title "Study on Regeneration System and Transgenic Impact Factors of Chinese Cabbage" @default.
- W2394055027 hasPublicationYear "2013" @default.
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