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- W2394062004 abstract "AIM To determine the effect of the thermal transformation on characteristic peaks of Honghua Injection(Carhtami Flos) by HPLC/UV/MS initially.METHODS When comparing ultrasonic extraction of Carthami Flos with heat extraction,the change in their characteristic peaks was observed in contrast with peaks of Honghua Injection.Separation was performed on an Alltima C18(250 mm×4.6 mm,5 μm) analytical column with mobile phase consisting of 0.2% acetic acid and acetonitrile in gradient elution mode at the flow rate of 0.8 mL/min.The UV detection wavelength was set at 310 nm and 403 nm.An electrospray mass spectrometer was utilized for qualitative analysis with positive and negative ion first order scan mode.The scanning zone ranged from 200 to 1 200.RESULTS Four characteristic peaks on the HPLC fingerprints of thermal transformation of Carthami Flos were indicated,peaks of hydroxyl-saflor yellow and its isomer in 10 min and 21 min were heat-stable,the peak of aurone in 42 min was heat-labile,and peak of chalcone in 33 min was generated by thermo-dissociation.CONCLUSION The method reveals there exist the thermal stabilized and heat-labile components,the latter induced by heat transformation,in Honghua Injection and Carthami Flos." @default.
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- W2394062004 date "2011-01-01" @default.
- W2394062004 modified "2023-09-23" @default.
- W2394062004 title "Thermal transformation characteristic peaks of Honghua Injection by HPLC/UV/MS" @default.
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