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- W2394072200 abstract "AIM Valproate (VPA) is widely used to treat epilepsy. Long-term treatment of women with VPA has been reported to be associated with a PCOS-like syndrome. The aim of the present study was to investigate the effects of clinically relevant concentrations of valproate on steroidogenesis and steroidogenesis acute regulatory protein (StAR),cholesterol side-chain cleavage enzyme (P450scc)and cytochrome P450 aromatase (P450arom) mRNA expression in human luteinized granulosa cells in vitro. METHODS Human luteinized granulosa cells were isolated from oocyte retrieval of in vitro fertilization procedure and were cultured with DMEM medium and treated with various concentrations of valproate (0, 100, 250 mg·L -1), the culture media was collected after 2 days for progesterone and estradiol measurements by standard radiommunoassay, StAR, P450scc and P450arom mRNA in granulose cells were detected by fluorescent real-time RT-PCR (TaqMan assay). RESULTS Valproate caused significant increase of progesterone (P0.05) and estradiol(P0.01) production, and significantly increasein StAR (P0.01), P450scc(P0.05) and P450arom (P0.01) mRNA expression were observed in cultured cells. CONCLUSION Valproate directly altered steroidognesis of steroidogenesis cells: valproate increases progesterone and estradiol secretion by inducing StAR, P450scc and P450arom mRNA expression in human luteinized granulosa cell. And the occurrence of polycystic ovaries and endocrine disorders in women taking valproate for epilepsy may be attributed to valproate itself. It also implies that environmental chemicals may be related to the pathogenesis of PCOS." @default.
- W2394072200 created "2016-06-24" @default.
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- W2394072200 date "2004-01-01" @default.
- W2394072200 modified "2023-09-26" @default.
- W2394072200 title "Effects of valproate on steroid production and correlative steroidogenesis enzyme mRNA in human luteinized granulose cells" @default.
- W2394072200 hasPublicationYear "2004" @default.
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