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- W2394072846 abstract "Purpose To investigate the effect of XIAP,Omi/HtrA2 and mifepristone on the apoptosis of HeLa cells,and to explore the interaction of XIAP and Omi/HtrA2.Methods Expression,intracellular localization and function of the XIAP,Omi/HtrA2 in HeLa cells were observed by confocal microscopy after transfection.HeLa cells were induced by mifepristone.At the same time,the apoptosis were analyzed for different groups by flow cytometry.The interaction of XIAP and Omi/HtrA2 were analyzed by confocal microscopy.Results (1)HeLa cells induced by mifepristone showed partly apoptosis(46.16%).But,HeLa cells transfected with pDsRed2-XIAP and induced by mifepristone showed 26.41% apoptosis rate.HeLa cells transfected with pDsRed2-Omi/HtrA2 and induced by mifepristone showed 78.85% apoptosis rate.(2)Confocal microscopy detection showed XIAP and Omi/HtrA2 resonance energy transfer phenomena in HeLa cells.Conclusion Mifepristone can promote apoptosis of HeLa cells.The DsRed2-XIAP fusion protein can inhibit apoptosis of HeLa cells by inducing of mifepristone.The DsRed2-Omi/HtrA2 fusion protein can promote apoptosis of HeLa cells by inducing of mifepristone.We demonstrate that the EGFP-Omi/HtrA2 and DsRed2-XIAP fusion protein exist interaction in living HeLa cell." @default.
- W2394072846 created "2016-06-24" @default.
- W2394072846 creator A5053389067 @default.
- W2394072846 date "2012-01-01" @default.
- W2394072846 modified "2023-09-26" @default.
- W2394072846 title "The effects of fluorescent protein recombinant plasmid with XIAP and OMI/HTRA2 on HeLa cells" @default.
- W2394072846 hasPublicationYear "2012" @default.
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