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- W2394076716 abstract "Objective To establish a quick method for determination of amino acids in the spinal cord and brain. Methods The concentration of amino acids was measured with reversed-phase high performance liquid chromatography, o-phthaldialdehyde and β-mercaptoethanol precolumn derivatization and fluorescent detection (E_x=280 nm,E_m=340 nm). The mobile system is 0.1 mol/L potassium phosphate buffer (pH=(6.00)-(6.25) containing 46% methanol and 2% tetrahydrofuran with a flow rate of 1.0 ml/min. Results The linear range of the detection was 0.1～40.0 μmol/L, the mean correlation coefficient between the peak area and amino acid concentration was 0.999 2. The coefficient of variation within-day and between-day were 2.41%-5.23% and 3.51%-6.48% respectively. The mean recovery rate of amino acids could reach 90.13%-98.47%. Conclusions The method is simple, rapid and sensitive, and can be used for measurement of the concentration of amino acid in the spinal cord and brain." @default.
- W2394076716 created "2016-06-24" @default.
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- W2394076716 date "2005-01-01" @default.
- W2394076716 modified "2023-09-24" @default.
- W2394076716 title "Rapid determination of amino acids in the spinal cord and brain by high performance liquid chromatography with fluorescent detection" @default.
- W2394076716 hasPublicationYear "2005" @default.
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