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- W2394080657 abstract "The soil microbial DNA was directly extracted from aeolian sandy soil. The DNA crude extracts were obtained by the cell lysis via the repeated freezing-thawing process of SDS,lysozyme,CTAB,proteinase K and liquid nitrogen in combination with the crude extracts purified by PEG (polyethylene glycol). In this way the large fragment DNA was obtained from the lytic cells with the DNA yield increased,i.e.,0.586～1.311 μg·g-1(dry soil). In addition,the fragment size of DNA was over 23 Kb,and PEG was helpful to remove humic acid in DNA effectively. The unpurified DNA could be directly used as templates for PCR amplification of 16S rDNA. The method mentioned above was simple and appropriate for DNA extraction from the aeolian sandy soil." @default.
- W2394080657 created "2016-06-24" @default.
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- W2394080657 date "2010-01-01" @default.
- W2394080657 modified "2023-09-23" @default.
- W2394080657 title "A Method for Total Microbial DNA Extraction Directly Meeting the Requirement of PCR Amplification from an Aeolian Sandy Soil" @default.
- W2394080657 hasPublicationYear "2010" @default.
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