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- W2394099260 abstract "Objective To investigate the clinical value of StaphPlex and xMAP technology for rapid identification of Staphylococcus and detection of antibiotic resistant genes from gram-positive cocci in blood cultures.Methods After gram-positive cocci in clusters were observed in blood cultures,StaphPlex system and xMAP technology were used for phenotypic identification of Staphylococcus and detection of antibiotic resistant genes.The results were compared with those of routine identification and susceptibility testing.SCCmec and PVL genes of four methicillin-resistant Staphylococcus aureus(MRSA) were detected with StaphPlex and real-time fluorescence quantitative PCR respectively.Results The positive rate of Staphylococcus was 91.3%(21/23),and that of non-Staphylococcus was 8.7%(2/23).The routine phenotypic identification demonstrated that one of the two non-Staphylococcus isolates was Staphylococcus capitis,and the other was Enterococcus faecium.The results of detection of aacA,ermA,ermC,SCCmec and the PVL gene by StaphPlex were highly consistent with those of drug sensitivity tests in vitro and real-time fluorescence quantitative PCR.Conclusions StaphPlex is a new technology for rapidly and accurately detecting Staphylococcus in blood cultures and drug resistance genes,and can be used in clinical diagnosis." @default.
- W2394099260 created "2016-06-24" @default.
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- W2394099260 date "2010-01-01" @default.
- W2394099260 modified "2023-09-23" @default.
- W2394099260 title "Combination of StaphPlex and xMAP technology for rapid identification of Staphylococcus and detection of antibiotic resistant genes in blood cultures" @default.
- W2394099260 hasPublicationYear "2010" @default.
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