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- W2394138196 abstract "The present work was aimed to investigate the immune efficiency of the recombinant subunit vaccine of goose parvovirus(GPV).Both of VP1and VP3genes were amplified from genome DNA of ZJ-04 strain,the amplified fragments were cloned into pMD-18 Tvector and sequenced subsequently.The VP3 gene was then subcloned into the expression plasmid pET-32a(+),and the recombinant plasmid pET-VP3 was used to transform Ecoli BL-21(DE3)competent cells and the recombinant bacteria BL-VP3was obtained.20 28-week-old breeding Shitou geese were inoculated with oil emulsion vaccine prepared with recombinant VP3inclusion or BL-VP3respectively,and boosted 2weeks later.The serum GPV antibody titers of the birds were detected by agar diffusion test,and the mortality of the goslings challenged with ZJ04was observed to evaluate the immune protection efficiency of GPV maternal antibody.The results revealed that the VP1gene of ZJ-04was consisted of 2 199bp,encoding for 732amino acids,and the nucleotide sequence homology among ZJ-04and other reference GPV strains recorded in Genbank were 95.3%99.2%,and the homology of the deduced amino acid sequence also came to 97.7%-99.3%.SDS-PAGE and Western-blot detection showed that the recombinant VP3,with molecular weight of 80ku,could specifically reacted with GPV antisera.The immunized geese inoculated with VP3inclusion or BL-VP3vaccine showed higher serum antibody titers against GPV,and the goslings originated from the immunized geese above showed perfect resisitant to the challenge of ZJ-04." @default.
- W2394138196 created "2016-06-24" @default.
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- W2394138196 date "2013-01-01" @default.
- W2394138196 modified "2023-09-23" @default.
- W2394138196 title "Preparation and Immune Effect of VP3 Subunit Vaccine of Goose Parvovirus" @default.
- W2394138196 hasPublicationYear "2013" @default.
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