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- W2394167923 abstract "Objective Building sugars marker carrier vector for screening clone.Methods Used pPIC9K-βGl2 as a template,amplified Bgl2 full-length gene fragment by PCR,cloned into pET-28a vector and transformed into E.coli BL21(DE3),screened clones by Cel-M9 medium.The recombinant pET-28a-Bgl2 expression plasmids were successfully constructed and analyzed by DNA sequencing.From the result of the SDS-PAGE,the recombinant plasmid could express the target protein after IPTG inducment,its molecular weight met the expectation.Result Got Bgl2 gene fragments by PCR amplification,SDS-PAGE analysis showed that the protein expressed as inclusion as inclusion body,and positive clones could screened by Cel-M9 medium.Conclusion Screened clones by Cel-M9 medium and built the foundation for food-grade vectors." @default.
- W2394167923 created "2016-06-24" @default.
- W2394167923 creator A5056009333 @default.
- W2394167923 date "2012-01-01" @default.
- W2394167923 modified "2023-09-25" @default.
- W2394167923 title "Cloning and expression of aspergillus niger β-Glucosidase gene in escherichia coli" @default.
- W2394167923 hasPublicationYear "2012" @default.
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